The US AI ecosystem remained exceptionally strong in 2025. According to a TechCrunch report listing startups that raised $100 million or more, 55 companies across healthcare, legal tech, generative media, AI infrastructure, and hardware secured major funding rounds throughout the year. Investors backed early-stage and growth-stage companies alike, reflecting broad confidence in AI’s expansion across industries.
January
Hippocratic AI (healthcare AI startup): $141M Series B, $1.6B valuation. Led by Kleiner Perkins; Andreessen Horowitz, Nvidia, General Catalyst participated.
ElevenLabs (synthetic voice AI): $180M Series C, $3B+ valuation. Led by Iconiq, Andreessen Horowitz; Sequoia, NEA, Salesforce Ventures participated.
February
Together AI (open-source generative AI infrastructure): $305M Series B, $3.3B valuation. Co-led by Prosperity7, General Catalyst; Salesforce Ventures, Nvidia, Lux Capital participated.
Lambda (AI infrastructure & model development): $480M Series D, ~$2.5B valuation. Co-led by SGW, Andra Capital; Nvidia, G Squared, ARK Invest participated.
Abridge (healthcare AI transcription platform): $250M Series D, $2.75B valuation. Co-led by IVP, Elad Gil; Lightspeed, Redpoint, Spark Capital participated.
Harvey (legal AI startup): $300M Series D, $3B valuation. Led by Sequoia; OpenAI Startup Fund, Kleiner Perkins, Elad Gil participated.
Eudia (AI legal tech): $105M Series A. Led by General Catalyst; Floodgate, Defy Ventures, Everywhere Ventures participated.
EnCharge AI (AI hardware startup): $100M Series B. Led by Tiger Global; Scout Ventures, Samsung Ventures, RTX Ventures participated.
March
Anthropic (AI research & LLMs): $3.5B Series E, $61.5B valuation. Led by Lightspeed; Salesforce Ventures, Menlo Ventures, General Catalyst participated.
OpenAI (large language models & AI research): $40B funding round, $300B valuation. Led by SoftBank; Microsoft, Thrive Capital, Coatue participated.
Nexthop AI (AI infrastructure company): $110M Series A. Led by Lightspeed Venture Partners; Kleiner Perkins, Battery Ventures, Emergent Ventures participated.
Insilico Medicine (AI drug discovery platform): $110M Series E, $1B valuation. Co-led by Value Partners, Pudong Chuangtou.
Celestial AI (AI infrastructure startup): $250M Series C, $2.5B valuation. Led by Fidelity; Tiger Global, BlackRock, Intel CEO Lip-Bu Tan participated.
Lila Sciences (science superintelligence platform): $200M seed. Led by Flagship Pioneering; March Capital, General Catalyst, ARK Venture Fund participated.
Reflection AI (autonomous AI research): $130M Series A, $580M valuation. Led by Lightspeed, CRV.
Turing (developer AI platform): $111M Series E, $2.2B valuation. Led by Khazanah Nasional; WestBridge Capital, Gaingels, Sozo Ventures participated.
Shield AI (AI defense tech startup): $240M Series F, $5.3B valuation. Co-led by L3Harris Technologies, Hanwha Aerospace; Andreessen Horowitz, US Innovative Technology Fund participated.
April
SandboxAQ (AI for science & climate): $450M Series E, $5.7B valuation. Nvidia, Google, Ray Dalio participated.
Runway (generative AI for media production): $308M Series D, $3B valuation. Led by General Atlantic; SoftBank, Nvidia, Fidelity participated.
May
Snorkel AI (data labeling & model training): $100M Series D, $1.3B valuation. Led by Addition; Lightspeed, Greylock, Prosperity7 Ventures participated.
LMArena (AI benchmarking tool): $100M seed, $600M valuation. Co-led by Andreessen Horowitz, UC Investments; Lightspeed, Kleiner Perkins, Felicis participated.
TensorWave (AI infrastructure & compute): $100M Series A. Co-led by Magnetar Capital, AMD Ventures; Nexus Venture Partners, Prosperity7 Ventures, Maverick Silicon participated.
June
Abridge (healthcare AI transcription): $300M Series E, $5.3B valuation. Led by Andreessen Horowitz; Khosla Ventures participated.
Harvey (legal AI startup): $300M Series E, $5B valuation. Co-led by Kleiner Perkins, Coatue.
Tennr (healthcare AI startup): $101M Series C, $605M valuation. Led by IVP; Lightspeed, GV, Andreessen Horowitz participated.
Glean (enterprise AI search): $150M Series F, $7.25B valuation. Led by Wellington; Sequoia, Lightspeed, Kleiner Perkins participated.
Anysphere (AI research lab – Cursor): $900M Series C, ~$10B valuation. Led by Thrive Capital; Andreessen Horowitz, Accel, DST Global participated.
July
Fal (generative media platform): $125M Series C, $1.5B valuation. Led by Meritech Capital Partners; Salesforce Ventures, Shopify Ventures, Google AI Futures Fund participated.
Ambience Healthcare (AI healthcare operating system): $243M Series C. Led by Oak HC/FT, Andreessen Horowitz; Kleiner Perkins, OpenAI Startup Fund, Smash Capital participated.
Reka AI (AI research lab): $110M Series B, $1B valuation. Snowflake, Nvidia participated.
Thinking Machines Lab (AI research lab): $2B seed, $12B valuation. Led by Andreessen Horowitz; Nvidia, Accel, AMD participated.
OpenEvidence (AI-powered clinician search tool): $210M Series B, $3.5B valuation. Led by Kleiner Perkins, GV.
Harmonic (mathematical reasoning engine AI): $100M Series B, $875M valuation. Led by Kleiner Perkins.
August
EliseAI (healthcare & housing automation AI): $250M Series E, ~$2.2B valuation. Led by Andreessen Horowitz.
Decart (AI research lab): $100M funding, $3.1B valuation. Sequoia, Benchmark, Zeev Ventures participated.
September
Periodic Labs (AI scientist startup): $300M seed. Led by Felicis, Andreessen Horowitz; Nvidia, Lightspeed, Khosla Ventures participated.
Cerebras Systems (AI hardware & infrastructure): $1.1B Series G, $8.1B valuation. Co-led by Fidelity, Atreides Management.
Modular (AI infrastructure startup): $250M funding. Led by US Innovative Technology Fund; GV, Greylock, General Catalyst participated.
Distyl AI (enterprise AI software): $175M Series B, $1.8B valuation. Led by Khosla Ventures, Lightspeed.
Upscale AI (AI infrastructure startup): $100M seed. Co-led by Maverick Silicon, Mayfield; StepStone Group, Stanford University, Qualcomm Ventures participated.
Groq (AI inference hardware startup): $750M Series D-3, $6.9B valuation. Led by Disruptive.
Invisible Technologies (AI training startup): $100M funding, $2B valuation. Led by Vanara Capital; Greycroft, Tallwoods Capital, Freestyle Capital participated.
Cognition AI (vibe-coding agent Devin): $400M Series C, $10.2B valuation. Led by Founders Fund.
Baseten (AI infrastructure startup): $150M Series D, $2.1B valuation. Led by Bond; CapitalG, IVP, Spark Capital participated.
Sierra (customer service AI platform): $350M, $10B+ valuation. Led by Greenoaks Capital.
You.com (personalized AI search engine): $100M Series C, $1.5B valuation. Led by Cox Enterprises.
Anthropic (AI research & LLMs): $13B Series F, $183B valuation. Led by Iconiq, Fidelity, Lightspeed.
October
Fireworks AI (AI app platform using open-source models): $250M Series C, ~$4B valuation.
Uniphore (enterprise conversational AI): $260M Series F, $2.5B valuation. Led by Snowflake Ventures, Nvidia, Databricks Ventures.
Sesame (voice AI company): $250M Series B. Co-led by Sequoia, Spark Capital; SignalRank participated.
OpenEvidence (AI medical chatbot): $200M Series C, $6B valuation.
Lila Sciences (science superintelligence platform): $350M Series A. Co-led by Braidwell, Collective Global.
Reflection AI (DeepSeek competitor): $2B Series B, $8B valuation. Led by Nvidia.
EvenUp (legal AI startup): $150M Series E, $2B+ valuation. Led by Bessemer; Lightspeed, Bain Capital, SignalFire participated.
November
Genspark (all-in-one AI workspace): $275M Series B, $1.25B valuation. Emergence Capital Partners, SBI Investment, LG Technology Ventures participated.
Luma AI (generative media platform): $900M Series C, $4B valuation. Led by Humain; Andreessen Horowitz, AMD Ventures, Amplify Partners participated.
Anysphere (AI coding tool – Cursor): $2.3B funding, $29.3B valuation. Second round this year.
Parallel (web infrastructure for AI agents): $100M Series A. Co-led by Index Ventures, Kleiner Perkins.
Hippocratic AI (healthcare AI agents): $126M Series C, $3.5B valuation. Led by Avenir Growth; second round this year.
December
Mythic (AI hardware startup): $125M venture round. Led by DCVC; SoftBank, NEA, Linse Capital participated.
Chai Discovery (AI for biotech/drug discovery): $130M Series B, $1.2B valuation. Led by Oak HC/FT, General Catalyst.
Fal (generative media platform): $140M Series D, $4.5B+ valuation. Led by Sequoia.
Unconventional AI (computing architecture startup): $475M seed, ~$4.5B valuation. Led by Lightspeed, Andreessen Horowitz.
7AI (cybersecurity AI agents): $130M Series A. Led by Index Ventures; Greylock, Spark Capital, CRV participated.
Despite a few “mega-rounds” dominating headlines, 2025 showed that AI funding is broad, deep, and diverse. From healthcare and legal tech to infrastructure, generative media, and hardware, investors poured billions into companies at every stage, signaling continued confidence in AI’s transformative potential. As we move into 2026, early bets like Elon Musk’s xAI and Sam Altman’s Merge Labs suggest this momentum is far from slowing, with the next wave of breakthroughs and unicorns already on the horizon.